Distribution of 15 Human Kallikreins in Tissues and Biological Fluids

Distribution of 15 human kallikreins in tissues and biological fluids.

BACKGROUND Kallikreins (KLKs) are a group of 15 secreted serine proteases. Some KLKs are established or candidate cancer biomarkers, but for most the physiological function is unknown. We characterized the protein and mRNA abundance patterns of all 15 KLKs in multiple panels of human tissues and biological fluids. METHODS We used sensitive and specific sandwich-type ELISAs for each KLK. Rever...

Development of an immunofluorometric assay for human kallikrein 15 (KLK15) and identification of KLK15 in tissues and biological fluids.

BACKGROUND Human kallikrein 15 (KLK15) may have some utility as a prostate, ovarian, and breast cancer biomarker, based on previous studies, which examined mRNA levels of KLK15. The aim of this study was to develop analytical technology for human kallikrein 15, including recombinant protein, specific antibodies, and a sensitive and specific ELISA immunoassay. The assay was then used to examine ...

The Micro-Determination of Gold in Biological Fluids and Tissues.

The increasing interest in the use of gold salts as chemotherapeutic agents in the treatment of certain diseases (Hartfall, et al., 1937) has made apparent the necessity of an accurate, specific, and sensitive method for the determination of gold in various biological materials. Moreover, the lack of information regarding the metabolism and pharmacology of this element further emphasizes this n...

Distribution of Paroxetine in Postmortem Fluids and Tissues

Immunofluorometric assay of human kallikrein 10 and its identification in biological fluids and tissues.

BACKGROUND The human kallikrein 10 gene [KLK10, also known as normal epithelial cell-specific 1 gene (NES1)] is a member of the human kallikrein gene family. The KLK10 gene encodes for a secreted serine protease (hK10). We hypothesize that hK10 is secreted into various biological fluids and that its concentration changes in some disease states. The aim of this study was to develop a sensitive a...